Botulinum Neurotoxin Subtype A4 Originating from Nontoxigenic Clostridium botulinum
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Botulinum Neurotoxin Subtype A4 Originating from Nontoxigenic Clostridium botulinum.
In this issue of Applied and Environmental Microbiology (AEM), we publish a paper by Marite Bradshaw, William H. Tepp, Regina C. M. Whitemarsh, Sabine Pellett, and Eric A. Johnson from the Department of Bacteriology, University of Wisconsin— Madison (UW—M), reporting on the purification and characterization of subtype A4 botulinum neurotoxin (1). The authors describe the purification of recombi...
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Clostridium botulinum subtype A4 neurotoxin (BoNT/A4) is naturally expressed in the dual-toxin-producing C. botulinum strain 657Ba at 100× lower titers than BoNT/B. In this study, we describe purification of recombinant BoNT/A4 (rBoNT/A4) expressed in a nonsporulating and nontoxigenic C. botulinum expression host strain. The rBoNT/A4 copurified with nontoxic toxin complex components provided in...
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Botulinum neurotoxins (BoNTs) naturally exist as components of protein complexes containing nontoxic proteins. The nontoxic proteins impart stability of BoNTs in the gastrointestinal tract and during purification and handling. The two primary neurotoxin complexes (TCs) are (i) TC1, consisting of BoNT, nontoxin-nonhemagglutinin (NTNH), and hemagglutinins (HAs), and (ii) TC2, consisting of BoNT a...
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BACKGROUND Clostridium botulinum produces seven distinct serotypes of botulinum neurotoxins (BoNTs). The genes encoding different subtype neurotoxins of serotypes A, B, F and several dual neurotoxin-producing strains have been shown to reside on plasmids, suggesting that intra- and interspecies transfer of BoNT-encoding plasmids may occur. The objective of the present study was to determine whe...
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Daniel Hauser, Melvin W.Eklund, Hisao Kurazono, Thomas Binz, Heiner Niemann, D.Michael Gill, Patrice Boquet and Michel R.Popoff* Unite des Anaerobies and 1 Unite des Antigenes Bacteriens, Institut Pasteur, 28 rue du Dr. Roux-75724 Paris cedex 15, France, Northwest and Alaska Fisheries Center, 2725 Montlake Boulevard East, Seattle, WA 98112, USA, lnstitut fur Mikrobiologie an der Bundesforschung...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2014
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.03001-14